Products & Services for Research

Maize growth response to 2 productsWhether you are from a research lab, or from a commercial branch, you probably should know about the quality of the product(s) you intend to use or sell. In the example at the right, one product resulted in a strong increase in fresh weight of maize, whereas the other one had no effect at all. However, the growth effect shown was not mediated by AM fungi, but by other effects. Studying products by similar assays gives us indication about the activity of AM fungi, but also about nutrient (fertilizer) content of products.

Note for your application that early plant responses may be dose-dependent; products with low concentration of AM fungi are not expected to show any AM fungal mediated effects after short times of 2-3 weeks, as AM fungi need some time to establish symbiosis and propagate.

All prices below are exclusive value added tax (VAT). You may inquire for a quotation for, e.g, larger sample quantities or assay numbers.
Please contact us for any inquiries by email to support@symplanta.com.

Products

We have reduced our portfolio of AM fungal inocula to the fungus that is used in vast majority of research approaches.

We offer research-grade quality AM fungal inoculum based mainly on fungal spores as propagules, a low number of root fragments may also be present. If you need material for larger scale research attempts (e.g. for field experiments), please inquire whether more economical but less well defined bulk standard inoculum quality is available. All material is produced aseptically, in vitro.  We supply AMF propagules in fine clay-based (calcined attapulgit) or diatom earth powder. Specific formulations like in liquid or in gel may be produced and delivered on demand, but this may take several months.  

Arbuscular mycorrhizal (AM) fungi, spores-based inoculum, in vitro produced (contamination free), research grade, we deliver a new formulation from July 2018:
 

Rhizophagus irregularis (syn: Glomus irregulare) SYMPLANTA-001 research grade (this 'model AM fungus' often was wrongly named 'Glomus intraradices' and corresponds to the fungal culture-line known as DAOM197198, DAOM181602 and MUCL43194 [note: although usually used in the sense of a culture number, DAOM numbers in fact are voucher numbers]. 1 million spores), supplied in 250 g water-insoluble fine powder (new formulation 2018).
00101SP, price: 250,- €

Rhizophagus irregularis (syn: Glomus irregulare) SYMPLANTA-001 research grade (this 'model AM fungus' often was wrongly named 'Glomus intraradices' and corresponds to the fungal culture-line known as DAOM197198, DAOM181602 and MUCL43194 [note: although usually used in the sense of a culture number, DAOM numbers in fact are voucher numbers]. 5 million spores), supplied in 1250 g water-insoluble fine powder (new formulation 2018).
00105SP, price: 1150,- €

Rhizophagus irregularis (syn: Glomus irregulare) SYMPLANTA-001 research grade (this 'model AM fungus' often was wrongly named 'Glomus intraradices' and corresponds to the fungal culture-line known as DAOM197198, DAOM181602 and MUCL43194 [note: although usually used in the sense of a culture number, DAOM numbers in fact are voucher numbers]. 10 million spores), supplied in 2500 g water-insoluble fine powder (new formulation 2018).
00110SP, price: 2250,- €

Carrier material, heat sterilized (e.g. for dilution or control experiments), we deliver a new formulation from July 2018:
 

Carrier material 1 kg.
The material is identical to the carrier in the powder based products. It can, e.g., be use for control experiments or dilution of inoculum. Only sold in combination with SYMPLANTA inoculum. Material is sterilized, but not packed under sterile conditions. If you need fully sterile material, please consider to heat-sterilize in your laboratory.
00001CA, price: 25,- €

Carrier material 5 kg.
The material is identical to the carrier in the powder based products. It can, e.g., be use for control experiments or dilution of inoculum. Only sold in combination with SYMPLANTA inoculum. Material is sterilized, but not packed under sterile conditions. If you need fully sterile material, please consider to heat-sterilize in your laboratory.
00005CA, price: 100,- €

Services

Species monitoring - NGS (MiSeq) based

Because of the stop of 454-sequencing support by Roche we adopted our system to the MiSeq platforms and already sucessfully conducted a number projects since June 2017.  
Please inquire well in advance for any related projects, as we may need about 3 months for individual projects, from start = order and pre-payment date.

DNA based high throughput species identification 'MiSeq' from various (field roots, culture substrate, etc.) samples.
PCR in triplicate, pooling, 450-500 bp based identification of AM fungi at species level, using a well established marker in the LSU rRNA gene region.
AMFMiSeq, project-price: costs depend on sample number and type (DNA extraction, etc.), please inquire!

Species identification - DNA based

DNA based species identification 'basic' from individual spores or roots (material provides must be from an isolate [single species]).
Identification of AM fungi based on PCR product sequencing, one sequence of a 450-500 bp LSU rRNA gene region, including nucleotide polymorphisms.
AMFS010, price: 100,- €  

AM fungi phylogenetic analysis.
Phylogenetic/phylotaxonomic analysis of your sequences or of sequences produced by SYMPLANTA. Based on a high quality alignment using our reference sequence data.
Inquire for pricing of your project

Inoculum viability and efficiency tests

Bioassay for quantification of the % root length colonization (RLC, in %) efficiency of substrates, products (e.g., commercial), or soil.
Based on a standard procedure (inoculum application in 3-4 dilutions, 5 replicates, root staining, % root length colonization analyses 7 weeks after inoculation) using a selected lettuce variety as test plant. If you need testing of other target plants, please inquire. Note that for a complete testing we will need 8-9 weeks.
AMFE001, price: 400,- €

 

Currently we do NOT offer the following services

Inoculum viability and efficiency tests

Standardised test of the % of viable spores in substrates, products (e.g., commercial), or soil.
Based on a standard procedure (MTT staining, 4 replicates, estimating number of putatively viable spore content). Note: this is only applicable with suited substrates (microscopic counting has to be possible) and also suited AMF species. Also note, that MTT positive reaction is not in all cases a 100% proof vor viability - if you need to be sure about colonization potential, you should apply a bioassay. MTT can be efficiently used for process control and QC of defined formulations.
AMFE003, price: 150,- € As customers tend to over-intepret results of MTT tests, we only offer it for in vitro produced materials of Rhizophagus species, or similar ones.

Species identification - DNA based

DNA based species identification 'detailed', from individual spores or mixed material (spores, roots, soil, ...).
PCR, cloning, RFLP screening, and DNA sequencing based identification of AM fungi, including 10 diverse >1500 bp sequences.
Inquire for complex samples, which e.g. demand multiple initial PCR reactions (pooled before cloning), and screening of larger clone numbers (approx. additional costs 100 €, but depends on number of sequences to be provided).

AMFS020, price: 950,-
currently not available

Species identification - spore morphology based

AM fungi surveys from soil; spore morphology based. Analysis of one normal sample (up to 5 AM fungal species in difficult substrate; up to 8 AM fungal species in a easy to handle substrate; if your samples fall in the high complexity category, we will inform you before any analyses).
AM fungal spore abundance is determined by spore extraction from 50 ml soil, taxonomic identification, and counting of the different morphotypes.
Includes photographic documentation and vouchers.

AMFS001, price: 150,- €
 no longer available

AM fungi surveys from soil; spore morphology based. Analysis of one complex sample (more than 5 AM fungal species in a difficult substrate; more than 8 AM fungal species in a easy to handle substrate; if samples fall in the normal category, we would inform you before analyses).
AM fungal spore ab
undance is determined by spore extraction from 50 ml soil, taxonomic identification, and counting of the different morphotypes.
Includes photographic documentation and vouchers. Please note: we are not overinterpreting data. We will give you the identification as far as possible; if identification is unsure, we will record this (morphological identification down to species level is impossible for some AM fungi - for such difficult species a DNA based identification is needed).
AMFS002, price: 250,- € no longer available