Products & Services for Research
Note that early plant responses may be dose-dependent. Products with moderate concentration of AM fungi are not expected to show positive growth effects after short times of 2-3 weeks, as the fungi need some time to establish symbiosis, propagate, and promote plant growth. Very early symbiotic stages may even show transient growth depression, reflecting the carbon-invest of the plant.
All prices are exclusive value added tax (VAT). You may inquire for a quotation for larger sample quantities, assay numbers, or MiSeq projects by email to office@symplanta.com.
Place your orders
Please simply send your inquiries and/or orders by email to
office@symplanta.com. As
international customer have very variable demands regarding the
administrative processes, we are not setting up a web-based system for scientific customers. We need your invoice address, and
delivery address.
We also need your VAT registration number for tax-free delivery
within the EU.
Shipping and handling costs for DHL parcels up to 2 kg
are
10.00 € for Germany (for express sendings within
Germany see
https://www.dhl.de/de/privatkunden/preise/preise-national.html
and add 5.00 €) and
20.00 € for other EU countries (for express sendings within
EU see
https://www.dhl.de/de/privatkunden/preise/preise-international.html
and add 6.00 €).
Other countries shipping costs are 6.00 € plus the shipping costs
according to DHL
(https://www.dhl.de/de/privatkunden/preise/preise-international.html).
Additional handling, administrative and packing costs may apply for
oversees shipping.
Products
We have reduced our portfolio to the Rhizophagus irregularis isolate that is used as a model organism in the vast majority of research approaches.
Our research-grade AM fungal inoculum contains mainly fungal spores as propagules (a very low number of small root fragments may be present). All material is produced aseptically, in vitro. We supply AMF propagules in diatom earth ('diatomite') powder with 50% humidity content. If you need material for larger scale research attempts (e.g. field experiments), please inquire for more economical but less well defined standard-grade inoculum (root fragments and spores, not packed under aseptical conditions). Specific formulations like in liquid or in gel may be produced and delivered on demand, but this may take several weeks or months.
Arbuscular mycorrhizal (AM) fungi, spores-based inoculum, in vitro produced, research grade, new formulation from July 2018:
Rhizophagus irregularis (syn:
Glomus irregulare) SYMPLANTA-001 research grade
(this 'model AM fungus' often was wrongly named 'Glomus intraradices'
and corresponds to the fungal culture-line
known as DAOM197198,
DAOM181602 and MUCL43194). 1 million spores supplied in 100 g water-insoluble fine, humid (50%
water content) diatomite powder.
00101SP, price: 230,- €
Rhizophagus irregularis
(syn:
Glomus irregulare) SYMPLANTA-001
research grade
(this 'model AM fungus' often was wrongly named 'Glomus intraradices'
and corresponds to the fungal culture-line
known as DAOM197198,
DAOM181602 and MUCL43194). 5
million spores supplied in 5x100 g water-insoluble fine, humid (50%
water content) diatomite powder.
00105SP, price: 1035,- €
(10% discount)
Rhizophagus irregularis
(syn:
Glomus irregulare) SYMPLANTA-001
research grade
(this 'model AM fungus' often was wrongly named 'Glomus intraradices'
and corresponds to the fungal culture-line
known as DAOM197198,
DAOM181602 and MUCL43194).
10 million spores supplied in 10x100 g water-insoluble fine, humid (50%
water content) diatomite powder.
00110SP, price: 1840,- €
(20% discount)
Carrier material, heat sterilized (e.g. for dilution or control experiments) :
Carrier material 50 g
The material is identical to the carrier
in the powder based products, but contains no water (thus, apply half
the weight when compared to
the inoculum, for
control experiments). It can,
e.g., be use for control experiments or for dilution of inoculum.
Only sold to SYMPLANTA inoculum customers.
Material is gamma-irradiation sterilized, but re-packed and sealed
under non-sterile conditions.
00001CA, price: 10,- €
Services
Species monitoring - NGS (MiSeq) based
Please inquire well in advance, as we may
need up to 3 months for individual projects, dependent on sample
numbers and sequencing availablity.
We
may request a 50% pre-payment.
DNA based high throughput species identification 'MiSeq' from
various (field soil, roots, culture substrate, etc.)
samples.
PCR in triplicate, pooling, 450-500 bp
based identification of AM fungi at
species level,
using a well established marker in the LSU
rRNA gene region.
Full analysis based on QIIME2 and EPA, species
level, EP numbers and current AMF species list see
www.amf-phylogeny.com
AMFMiSeq###, price: see below are
for standard soil samples (homogenous, no need for grinding and
sieving).
Root samples requiring grindig of individual samples will
cause some extra costs, please inquire.
Service | cost per sample |
AMFMiSeq010 10-11 samples | 200,00 € |
AMFMiSeq012 12-23 samples | 175,00 € |
AMFMiSeq024 24-47 samples | 150,00 € |
AMFMiSeq048 48-95 samples | 100,00 € |
AMFMiSeq096 96-143 samples | 90,00 € |
Example prices soil sample analyses |
cost per sample | cost per project |
10 samples | 200,00 € | 2.000,00 € |
20 samples | 175,00 € | 3.500,00 € |
40 samples | 150,00 € | 6.000,00 € |
80 samples | 100,00 € | 8.000,00 € |
120 samples | 90,00 € | 10.800,00 € |
Species identification - DNA based
DNA based species identification 'basic' from individual
spores or roots (material provides must be from an isolate [single
species]).
Identification of AM fungi
based on PCR product (amplicon) sequencing, one sequence of
450-500 bp of the D2 region of the LSU rRNA gene,
PCR amplicon consensus sequence including nucleotide polymorphisms. This fragment usually
is sufficient for species identification using our database
(provided the species is characterized). A maximum likelyhood
phylogenetic analysis and a phylogenetic tree of the respective
genus including the query sequence is included.
AMFS010, price: 100,- €
AM fungi phylogenetic analysis.
Phylogenetic/phylotaxonomic analysis of your sequences or of
sequences produced by SYMPLANTA. Based on a high quality alignment
using our
own reference sequence database.
AMFS100, price: 100,- € for an analysis of up to 10 sequences in a
phylogenetic tree covering all AM fungal taxa characterized up to
the presence;
other prices depend on your question, please inquire for your project
Inoculum viability and efficiency tests
Bioassay for quantification of the % root length
colonization (RLC, in %) efficiency of substrates, products (e.g.,
commercial inocula), or soil.
Based on a standard procedure (inoculum application in 3
or 4 dilutions, 5
replicates each, root staining, % root length colonization analyses
5-7 weeks after inoculation)
using a selected lettuce
variety as test plant. If you need testing of other
target plants, please inquire. Note that we will need
about 8 weeks for the entire procedure.
AMFE001, price: 400,- €
Currently we do not offer the following services
Inoculum viability and efficiency tests
Standardised test of the % of viable spores in substrates,
products (e.g., commercial).
Based on a standard procedure (MTT staining,
4 replicates,
estimating number of putatively viable spore
content). Note: this is only applicable
with suited substrates (microscopic counting has to be possible) and
also suited AMF species. Also note, that MTT positive reaction is
not in all cases a 100% proof vor viability - if you need to be sure
about colonization potential, you should apply a bioassay. MTT can
be efficiently used for process control and QC of defined
formulations.
AMFE003, price:
150,- €
as
customers tend to over-intepret results of MTT tests, we only offer
it for certain reasonable approaches.
Species identification - DNA based
DNA based species identification 'detailed', from
individual spores or mixed material (spores, roots, soil, ...).
PCR, cloning, RFLP screening, and DNA sequencing based
identification of AM fungi, including 10 diverse >1500 bp sequences.
Inquire for complex samples, which e.g. demand multiple initial PCR
reactions (pooled before cloning), and screening of larger clone
numbers (approx. additional costs 100 €, but depends on number of
sequences to be provided).
AMFS020, price: 950,- €
currently not
available
Species identification - spore morphology based
AM fungi surveys from soil; spore morphology based.
Analysis of one normal sample (up to 5 AM fungal
species in difficult substrate; up to 8 AM fungal species in a
easy to handle substrate; if your samples fall in the high
complexity category, we will inform you before any analyses).
AM fungal spore abundance is determined by spore extraction from 50
ml soil, taxonomic identification, and counting of the different
morphotypes.
Includes photographic documentation and vouchers.
AMFS001, price:
150,- € no
longer
available
AM fungi surveys from soil; spore morphology based.
Analysis of one complex sample (more than 5 AM fungal
species in a difficult substrate; more than 8 AM fungal species in a
easy to handle substrate; if samples fall in the normal category, we
would inform you before analyses).
AM fungal spore abundance is determined by spore extraction from 50
ml soil, taxonomic identification, and counting of the different morphotypes.
Includes photographic documentation and vouchers. Please note: we
are not overinterpreting data. We will give you the identification
as far as possible; if identification is unsure, we will record this
(morphological identification down to species level is impossible
for some AM fungi - for such difficult species a DNA based
identification is needed).
AMFS002, price:
250,- € no
longer
available