Services catalog - Research

Maize growth response to 2 productsWhether you are from a research lab, or from a commercial branch, you probably should know about the quality of the product(s) you intend to use or sell. See an example at the right, of two products tested in parallel. One product resulted in a strong increase in fresh weight (maize), whereas the other product had no effect on biomass at all. However, the growth effect shown was not mediated by AM fungi, but by other effects. We study product-quality by such assays, which also give us indication about nutrient (fertilizer) content of products.

Note for your application that early plant responses are dose-dependent; products with low concentration of AM fungi are not expected to show any clear effects after short times of 2-3 weeks. AM fungi need some time to establish symbiosis.

All prices below are exclusive value added tax (VAT). You may inquire for a quotation for, e.g, larger sample quantities or assay numbers.

Please contact us for any inquiries by email to support@symplanta.com.

PLEASE NOTE that due to the stopping of support for the 454-sequencing by Roche (producer) we could not offer deep sequencing based community analyses until having had adopted our system to other platforms (June-July 2017). We are now able to offer DNA based AMF characterisation again, from July 2017 on. Please inquire in in advance for any related projects, as we are not yet in routine processing state.

Species identification - DNA based

DNA based species identification 'basic' from individual spores or roots (material provides must be from an isolate [single species]).
PCR product sequencing based identification of AM fungi, one sequence of a certain LSU rRNA gene region, including analysis of nucleotide polymorphisms.
AMFS010, price: 240,- €  

DNA based species identification 'detailed', from individual spores or mixed material (spores, roots, soil, ...).
PCR, cloning, RFLP screening, and DNA sequencing based identification of AM fungi, including 10 diverse >1500 bp sequences.
Inquire for complex samples, which e.g. demand multiple initial PCR reactions (pooled before cloning), and screening of larger clone numbers (approx. additional costs 100 €, but depends on number of sequences to be provided).
AMFS020, price: 950,- 

AM fungi phylogenetic analysis.
Complete phylotaxonomic analysis of your sequences or of sequences produced by SYMPLANTA. The price for one fungal species is much higher than for a set of samples, as for larger numbers the effort per species is reduced significantly and the data-analyses can be run more economically. We also provide you with a high quality alignment based on our reference sequence data.
Inquire for pricing of your project

Inoculum viability and efficiency tests

Standardised quantification of the % root length colonization (RLC, in %) efficiency of substrates, products (e.g., commercial), or soil.
Based on a standard procedure (inoculum application in 3 dilutions, 5 replicates, root staining, % root length colonization analyses 7 weeks after inoculation) using a selected lettuce variety as test plant. If you need testing of other target plants, please inquire. Note that for a complete testing we will need 8-9 weeks.
AMFE001, price: 400,- €

Standardised test of the % of viable spores in substrates, products (e.g., commercial), or soil.
Based on a standard procedure (MTT staining, 4 replicates, estimating number of putatively viable spore content). Note: this is only applicable with suited substrates (microscopic counting has to be possible) and also suited AMF species. Also note, that MTT positive reaction is not in all cases a 100% proof vor viability - if you need to be sure about colonization potential, you should apply a bioassay. MTT can be efficiently used for process control and QC of defined formulations.
AMFE003, price: 90,- €

 

Species identification - spore morphology based

no longer available from our lab.

AM fungi surveys from soil; spore morphology based. Analysis of one normal sample (up to 5 AM fungal species in difficult substrate; up to 8 AM fungal species in a easy to handle substrate; if your samples fall in the high complexity category, we will inform you before any analyses).
AM fungal spore abundance is determined by spore extraction from 50 ml soil, taxonomic identification, and counting of the different morphotypes.
Includes photographic documentation and vouchers.

AMFS001, price: 150,- €
 no longer available

AM fungi surveys from soil; spore morphology based. Analysis of one complex sample (more than 5 AM fungal species in a difficult substrate; more than 8 AM fungal species in a easy to handle substrate; if samples fall in the normal category, we would inform you before analyses).
AM fungal spore ab
undance is determined by spore extraction from 50 ml soil, taxonomic identification, and counting of the different morphotypes.
Includes photographic documentation and vouchers. Please note: we are not overinterpreting data. We will give you the identification as far as possible; if identification is unsure, we will record this (morphological identification down to species level is impossible for some AM fungi - for such difficult species a DNA based identification is needed).
AMFS002, price: 250,- € no longer available